Seahorse XFe-96

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Seahorse XFe-96

Seahorse XFe-96

Instrument category: Biochemistry Core

Instrument mode:

Unit: 浙江大学 > ZJU-UoE Institute

Instrument manufacturer: Agilengt

Purchase date: 2020-10-10

Usage patterns: Sample presentation reservation,Machine-time reservation

Specification: XFe-96

Room number:A107

Collection

Reservation

Instrument manufacturer: Agilengt

Asset number: 20031450

Asset manager: 林江涛

Purchase date: 2020-10-10

Instrument price: 170.00 Ten thousand yuan

Origin of instruments: American

Instrument supplier: 杭州宝诚生物技术有限公司

Purchase manager: 林江涛

Main accessories: ----

Main parameter: 96-well plate format accommodates many conditions in a single run, for flexible assay design, dose-response studies, and screening Reports real-time metabolic rates in minutes, without sample extraction or labelling Four-port injection system with automated mixing function, for detection of live-cell responses to substrates, inhibitors, and other compounds in real time High-sensitivity analysis of as few as 5,000 cells per well using the custom 96-well plate Precision-controlled heating tray, maintains 16–42°C temperatures (12–20°C above ambient conditions), for compatibility with a variety of sample types Quickly determine dependency of cellular energy production on mitochondrial substrates Generate a metabolic phenotype within one hour for fast data turnaround Analyze 3D samples such as spheroids and islets Wave software allows easy assay protocol creation and data analysis on your desktop PC and export to common spreadsheet and graphing programs

Introduction of instruments:

Agilent Seahorse XFe96 Analyzers measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate format.

OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis as well as ATP production rate. Together these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex vivo samples.

Working hours: 09:00-12:00;14:00-18:00
Minimum reservation time interval: 0.25 Hours
Maximum reservation time interval: 168 Hours
Minimum unit time of calendar: 0.25 Hours
Latest reservation time: Unauthorized user: 0Hours; General qualified user: 0Hours; Senior qualified user: 0Hours
Earlist reservation time: Unauthorized: 8 Week 0 o'clock; Ordinary: 8 Week 0 o'clock; Senior: 8 Week 0 o'clock
Reservation protection time: 30 minutes
No-show protection time: 30 minutes
Outage delay time: 30 Seconds
Minimum power-on time: 5 Seconds
Timeout reminder: 1200 Seconds
Maximum effective number of reservations: 5 次/ Day
Costless revocation of reservation time: 120 minutes

Main instrument manager: 李媛(邮箱:yuanli@intl.zju.edu.cn)

Instrument manager: 沈杭晨(邮箱:shc14124818@163.com)

Instrument manager: 贾玲燕(邮箱:lingyanjia@intl.zju.edu.cn)

Instrument manager: 刘娣(邮箱:diliu@intl.zju.edu.cn)

Instrument manager: 王依依(邮箱:21716013@zju.edu.cn)

Instrument manager: 金梦迪(邮箱:mengdijin@intl.zju.edu.cn)

Instrument manager: 周博诚(邮箱:bochengzhou@intl.zju.edu.cn)

Instrument manager: 朋德测试(邮箱:11111@qq.com)

Instrument manager: 蔡政(邮箱:675097016@qq.com)

Instrument manager: 任艺飞(邮箱:yifeiren@intl.zju.edu.cn)

Instrument manager: 朱乐(邮箱:11616028@zju.edu.cn)

User qualification Working hours Non working time
Unauthorized qualification Need to review Need to review
General qualification Avoid audit Avoid audit
Senior qualification Avoid audit Avoid audit
To be audited
Valid
Completed
Breach of contrac
Occupied
No. Title Creation time Operation
No. Title File number Creation time Operation
1 Keys to Success 1 2023-10-09 16:16:23 Download files
2 Seahorse Introductory Training 1 2023-10-09 16:15:44 Download files
3 The experimental procedure of Seahorse XFe96 (Chinese) 1 2023-10-09 16:00:42 Download files
4 user guide 12 2021-01-11 22:12:14 Download files
Title Training Video Creation time Operation
Instrument operation video of Seahorse XFe96 1696838308tlenb.mp4 2023-10-09 16:02:41 Detail