浙江大学国际联合学院（海宁国际校区）

Medical biological safety Back

Single-choice question

Question 1

Which of the following statements about the use of CO2 incubators are wrong:

A:Tissue culture dishes should be placed in plastic trays for incubation.

B:Culture bottles and dishes can be stacked

C:Regular cleaning and disinfection

D:Managed by people, check CO2 and pipeline every day to prevent air leakage, check temperature, etc.

Correct answer：

B

Question 2

Metal vessels contaminated with biological materials such as microorganisms cannot be disinfected using which one of the following solutions

A:2% glutaraldehyde

B:75% alcohol solution

C:1% bleach solution

D:High concentration soapy water

Correct answer：

C

Question 3

After the pathogen material is homogenized or stirred, which of the following places should the container be opened?

A:On the bench

B:Biological safety cabinet

C:Sterile room

D:Ultra-clean workbench

Correct answer：

B

Question 4

Immediately after use of the glass (bacterial) filter, which of the following solutions is used for suction filtration. When the washing solution has not been filtered out, the filter is immersed in the above washing solution for 48 hours (both sides of the filter should be contacted with the washing liquid).

A:1% hydrochloric acid

B:Potassium dichromate washing solution

C:Concentrated sulfuric acid-sodium nitrate washing solution

D:Sodium hydroxide or dilute sodium bicarbonate solution

Correct answer：

C

Question 5

Which of the following gas fumigants can be used for disinfection?

A:Formaldehyde

B:Ether

C:Ammonia

D:Ethanol

Correct answer：

A

Question 6

How long does it take to sterilize by fumigation and at least to keep the fumigation process closed?

A:12h

B:24h

C:6h

D:8h

Correct answer：

A

Question 7

What is the correct statement about the operation of the biosafety cabinet?

Correct answer：

D

Question 8

When filtering and sterilizing, the bacteria solution should be injected into the filter for filtration. The time should not be too long. How much is the pressure control?

A:0～30mmHg

B:100～200mmHg

C:200～500mmHg

Correct answer：

B

Question 9

When filtering and sterilizing operations, what equipment is used for disinfection and sterilization before use of filters and filter bottles?

A:Oven

B:Autoclave

C:Heating vacuum oven

D:Microwave oven

Correct answer：

B

Question 10

Put the sequence of filtration sterilization operations in the correct order: a. All equipment such as filters and filter bottles are packaged and autoclaved before use. b. Use sterile tubes to aseptically operate filter bottles, safety bottles, differential pressure gauges. Connect with the pumping system c. Inject the liquid to be filtered into the filter for filtration. The time should not be too long. The pressure is controlled to 100-200mmHg d. When used, install the filter on the filter bottle under aseptic conditions.

A:abcd

B:adcb

C:abdc

D:adbc

Correct answer：

D

Question 11

When immersing and disinfecting, water-soluble chemical disinfectants with wide bactericidal spectrum and weak corrosiveness are often used. Which of the following is most appropriate?

A:Bleaching powder (sodium hypochlorite)

B:Come to Suer (cresol)

C:Formalin (formaldehyde)

D:Glutaraldehyde

Correct answer：

A

Question 12

When opening the lyophilized ampule, some of the lyophilizate may spill due to pressure drop. In which facilities should this operation take place?

A:On the bench

B:Sterile room

C:Biological safety cabinet

D:Ultra-clean workbench

Correct answer：

C

Question 13

What glassware should be placed in the equipment for disinfection and sterilization?

A:Oven

B:Autoclave

C:Heating vacuum oven

D:Microwave oven

Correct answer：

B

Question 14

Where should the container be opened after homogenization or agitation of the experimental material involved in the pathogenic microorganism?

A:On the bench

B:Biological safety cabinet

C:Sterile room

D:Ultra-clean workbench

Correct answer：

B

Question 15

Which of the following is not a necessary disinfectant solution in biomedical laboratories?

A:0.5% sodium hypochlorite solution

B:1.0% of the comel solution

C:75% ethanol solution and cotton balls

D:10% ammonia water

Correct answer：

D

Question 16

Hygrothermal sterilization is the use of heat to sterilize, usually in what equipment?

A:High pressure steam sterilizer

B:Oven

C:High temperature water bath

D:Microwave oven

Correct answer：

A

Question 17

Which of the following requirements is unnecessary when using an autoclave?

A:The sterilizer cavity should be loosely loaded so that the steam acts evenly

B:Must wear protective clothing and gloves

C:The drain filter of the sterilizer should be removed and cleaned frequently

D:Turn on the autoclave when the temperature drops below 80 °C

Correct answer：

B

Question 18

Which of the following precautions regarding the use of autoclaves is incorrect:

A:Pay attention to protection, prevent burns, and open the cover after the temperature drops.

B:It is forbidden to sterilize the vessel with the lid, which is easy to burst

C:Regularly check drains

D:Fill an autoclaved item as much as possible

Correct answer：

D

Question 19

Which of the following operations is wrong when using the Biosafety Cabinet (BSC)?

A:The BSC fan should be operated for 5 minutes before starting work and after work. Pipettes or other substances cannot block the air grille in front of the work area.

B:No need for surface cleaning before the items are placed in the working area of the cabinet. The glass panel can be opened during use.

C:The experimental operation should be done at the middle and rear of the workbench

D:The operator should minimize the extension and removal of the arm

Correct answer：

B

Question 20

The mistakes made in the use of biological experimental materials are:

Correct answer：

C

Question 21

Which of the following is correct when using a pipette?

A:Repeated inhalation and extraction of infectious substances with pipettes

B:A pipette can be used to absorb a variety of solutions without washing

C:Can blow any liquid of infectious substances into the air

D:When no ear balls are washed, the mouth pipette cannot be used

Correct answer：

D

Question 22

Which of the following methods is used to sterilize contaminated pipettes, then rinse with tap water and deionized water?

A:Suitable disinfectant for soaking and autoclaving in the pot

B:In the autoclave

C:Soaking in a suitable disinfectant

D:Soaking in soapy water

Correct answer：

A

Question 23

What disinfectant solution should be used regularly to wipe walls, floors, tables, chairs and other facilities?

A:0.05% New Cleaner

B:95% ethanol

C:Potassium permanganate

D:Detergent

Correct answer：

A

Question 24

For harmful microbial and transgenic operations in biomedical laboratories, use:

A:Biological safety cabinet

B:Purification workbench

C:Ordinary test bench

D:Fume hood

Correct answer：

A

Question 25

UV disinfection is convenient and practical. The UV lamp can be fixed or movable, but should not be more than what distance from the object being irradiated?

A:1.2m

B:2 m

C:2.2 m

D:3.2m

Correct answer：

A

Question 26

The maximum temperature of the constant temperature incubator is:

A:60℃

B:100℃

C:45℃

D:80℃

Correct answer：

A

Question 27

What is the pressure in the animal laboratory of the normal barrier system compared to the outdoor?

A:Positive pressure

B:Negative pressure

C:Isobaric pressure

D:Random

Correct answer：

A

Question 28

If there is an accident in the microbiological experiment (such as broken seed tube), what should be used immediately to clean the table, wash hands, etc., in time to kill bacteria and viruses, to avoid the expansion of pollution?

A:Disinfectant (84 disinfectant)

B:Use ordinary tap water

C:Pure water

D:Ethanol

Correct answer：

A

Question 29

In microbial experiments, some unwanted strains, etc. can be:

A:Dump directly down the water pipe

B:Discarded directly after disinfection and autoclaving

C:After disinfection and autoclaving, discard it again (same as B?)

Correct answer：

C

Question 30

In microbiological experiments, some vessels that are contaminated or contain harmful bacteria and viruses can:

A:Reuse without disinfection and autoclaving

B:Discarded without disinfection and autoclaving

C:After disinfection and autoclaving, reuse

Correct answer：

C

Question 31

In which level of biosafety laboratory should the rabies virus culture or animal infection test be conducted?

A:BSL-1 or ABSL-1

B:BSL-2 or ABSL-2

C:BSL-3 or ABSL-3

D:BSL-4 or ABSL-4

Correct answer：

C

Question 32

What should be done to treat dead bodies and tissue fragments of dead animals in biomedical laboratories?

A:Drop it into a household trash can in a plastic bag.

B:Self-buried

C:Sealed in a special plastic bag, frozen and stored, and handed over to a specialized agency

D:Self-burning treatment

Correct answer：

C

Question 33

The experimental animals used for scientific research must be derived from the units or departments with what certification?

A:Laboratory Animal Production License

B:Laboratory animal use license

C:Laboratory Animal Environmental Facilities Certificate

D:Animal experiment use license

Correct answer：

A

Question 34

At present, which department of the school is the department that supplies qualified experimental animals or provides qualified animal experimental sites?

A:Laboratory Animal Center

B:Material Supply Center

C:Logistics department

D:Equipment Supply Department

Correct answer：

A

Question 35

Anyone who is engaged in animal experiments must obtain which form of proof from the relevant department?

A:Laboratory Animals Employment Certificate

B:Health certificate

C:Professional degree certificate

D:Animal Laboratory Technician Qualification Certificate

Correct answer：

D

Question 36

According to the national standards for laboratory biosafety, what sites should not engage in high pathogenic pathogenic microorganisms?

A:Primary and secondary laboratories

B:Level 3 laboratory

C:Level 4 Laboratory

Correct answer：

A

Question 37

Which of the following methods of disposing of the experimental suit is wrong?

A:When leaving the laboratory, the lab coat must be taken off and left in the lab.

B:Experimental clothes can be worn out

C:Used work clothes should be disinfected in the laboratory first, then uniformly washed or discarded

D:The experimental suit cannot be carried home

Correct answer：

B

Question 38

Personal protective equipment in biomedical laboratories does not include:

A:Masks, hats, disposable gloves

B:Protective glasses

C:Protective clothing

D:Paper towels

Correct answer：

D

Question 39

In the following notes on the secondary biosafety protection laboratory, the mistake is:

A:Special safety equipment such as biological safety cabinets must be used

B:Staff should wear overalls and protective glasses when testing

C:Workers should wear gloves only when the skin is damaged or rashed

D:Must have emergency protection facilities such as sprinklers and eye washes

Correct answer：

C

Question 40

Which of the following is not the purpose of laboratory biosafety protection?

A:Protect the tester from infection by the subject

B:Ensure that other laboratory personnel are not affected by the subject

C:Ensure that the surrounding environment is not contaminated

D:Guaranteed to get the ideal experimental results

Correct answer：

D

Question 41

When pipetting, when there is a bio-contaminating substance spilling, in order to prevent the spread:

A:Immediately treat with cloth or paper soaked in disinfectant and then autoclave the contaminated items.

B:Immediately cloth or paper treatment

C:Immediate use of autoclaving

D:.Immediately be treated with cloth or paper soaked in disinfectant

Correct answer：

A

Question 42

Dry heat sterilization use temperature and time are usually:

A:160-400℃，1-5h

B:160-400℃，15-30min

C:160-400℃，15-30min

Correct answer：

A

Question 43

Autoclaving is the most effective and reliable method of sterilizing experimental materials. To ensure proper sterilization, which of the following sets of conditions is most appropriate?

A:121 ° C, sterilization for 10 minutes

B:100 ° C, sterilization for 30 minutes

C:121 ° C, sterilized for 15 minutes

D:121 ° C, sterilized for 15 minutes

Correct answer：

C

Question 44

Regarding UV disinfection, which of the following statements are incorrect:

A:It can be fixed or movable, but it should be no more than 1.2m from the object to be irradiated.

B:UV disinfection is convenient and practical, but it cannot be relied upon for complete sterilization, especially for bacterial spores.

C:Ultraviolet rays have harmful effects on the human body, and can not be directly seen. You should not work under the open ultraviolet light

D:Ultraviolet rays can be used for disinfection of all pathogenic microorganisms

Correct answer：

D

Question 45

Spray disinfection is the use of aerosols formed from various chemical disinfectants to disinfect the surface of air and objects. Drugs that cannot be used for spray disinfection are:

A:2% glutaraldehyde

B:75% alcohol solution

C:Bleaching powder solution

D:Formaldehyde

Correct answer：

D

Question 46

Regarding the comparison between the two methods of moist heat sterilization and dry heat sterilization, the wrong statement is:

A:Wet heat sterilization is better than dry heat sterilization and is more common.

B:When using moist heat sterilization, the protein is easily denatured when it is rich in water, and it is easy to coagulate.

C:When using moist heat sterilization, the steam has latent heat. When the steam comes into contact with the sterilized item, it can condense into water and release latent heat, so that the temperature rises rapidly and the sterilization effect is enhanced.

D:Dry heat sterilization has stronger penetrating power than damp heat sterilization, and conduction is fast

Correct answer：

D

Question 47

What kind of pathogenic microorganisms do high-pathogenic avian influenza viruses and HIV (types I and II) belong to according to their degree of harm?

A:First class

B:Second category

C:Third category

D:Fourth category

Correct answer：

B

Question 48

Dry heat sterilization uses heat to sterilize, usually in which of the following equipment?

A:Microwave oven

B:Oven

C:High pressure steam sterilizer

D:Resistance furnace

Correct answer：

B

Question 49

The laboratory biosafety issue is not only related to the spread of diseases among animals, but also what is the main concern?

A:Success or failure of the experiment

B:Laboratory pollution

C:People health

D:Animal death

Correct answer：

C

Question 50

What is the best equipment for disinfecting and sterilizing equipment in animal laboratories?

A:Water heater

B:UV lamp

C:Ozone generator

D:Autoclave cabinet

Correct answer：

D

Question 51

In the experiments of molecular biology, genetics, genetic engineering, etc., which common drugs are carcinogens? The drug should not be allowed to come into contact with the skin during the experiment.

A:Monomer acrylamide

B:Methylene diacrylamide

C:Ethidium bromide (EB)

Correct answer：

C

Question 52

In the experiments such as genetics, which common drugs are carcinogens? You should not expose the drug to the skin during the experiment.

A:Monomeric acrylamide

B:Colchicine

C:Methylene diacrylamide

Correct answer：

B

Question 53

In biochemical protein electrophoresis experiments, which drug is a neurotoxic agent that, if exposed to the skin, will be absorbed?

A:Colchicine

B:Ethidium bromide (EB)

C:Methylene diacrylamide

Correct answer：

C

Question 54

What common substances in a biological laboratory are flammable and explosive liquids? It must be properly placed and used correctly.

A:Trinitrobenzenesulfonic acid, picric acid

B:Inorganic phosphorus

C:Ether, xylene, acetone, alcohol

Correct answer：

C

Question 55

What substances are flammable and explosive solids in a biological laboratory? It must be properly placed and used correctly.

A:Ethidium bromide (EB)

B:Rosin, sulfur, inorganic phosphorus

C:Trinitrobenzenesulfonic acid, picric acid

Correct answer：

B

True or False question

Question 1

When using a centrifuge, the centrifuge tube can be placed in the rotor without any consideration given to balancing.

A:TRUE

B:FALSE

Correct answer：

B

Question 2

During the centrifugation process, if you hear abnormal noise from the centrifuge, stop the machine and check the cause.

A:TRUE

B:FALSE

Correct answer：

B

Question 3

Each rotor of a high-speed centrifuge has its rated speed, and cannot be used beyond its rated speed.

A:TRUE

B:FALSE

Correct answer：

A

Question 4

When using the centrifuge, select a suitable centrifuge tube for the properties of the centrifuged liquid. In order to avoid cracking of the glass sleeve, the organic solvent and the enzyme solution are selected as plastic sleeves, and the salt solution is selected as a metal sleeve.

A:TRUE

B:FALSE

Correct answer：

B

Question 5

When operating biomaterials with a homogenizer, a homogenizer of Teflon material is recommended.

A:TRUE

B:FALSE

Correct answer：

A

Question 6

If the ampoule is stored in a liquid nitrogen tank, it should be stored in the vapor phase of liquid nitrogen.

A:TRUE

B:FALSE

Correct answer：

A

Question 7

When the autoclave liquid is terminated, the sterilizing liquid is taken out as quickly as possible, and the venting is quickly performed to rapidly reduce the pressure

A:TRUE

B:FALSE

Correct answer：

B

Question 8

When the autoclave is sterilized, the item to be sterilized may be sterilized together with a substance containing a corrosive inhibitor or a chemical agent.

A:TRUE

B:FALSE

Correct answer：

B

Question 9

Glassware contaminated with biological materials such as microorganisms should be autoclaved immediately and then cleaned.

A:TRUE

B:FALSE

Correct answer：

A

Question 10

When the experiment time is tight, you can work under the open UV lamp.

A:TRUE

B:FALSE

Correct answer：

B

Question 11

The isotope detector should be used to check the contamination status before entering the isotope laboratory and after the end of the experiment.

A:TRUE

B:FALSE

Correct answer：

A

Question 12

The waste generated by the pathogenic microorganisms experiment must be autoclaved before being classified and disposed of.

A:TRUE

B:FALSE

Correct answer：

A

Question 13

When a small amount of infectious material spills in the biosafety cabinet, apply the absorbent paper towel immediately, and immediately wipe the biosafety cabinet and its internal contents with a towel or gauze soaked with disinfectant. The gloves should be replaced after the work surface is disinfected, whether it is to remove the gloves or to change the gloves.

A:TRUE

B:FALSE

Correct answer：

A

Question 14

When a large amount of infectious material spills in the biosafety cabinet, the liquid will flow down through the grille in front of or behind the biosafety cabinet. All items in the biosafety cabinet should be surface disinfected and taken out of the biosafety cabinet. After ensuring that the drain valve of the biosafety cabinet is closed, the disinfectant can be poured onto the work surface to allow liquid to flow through the grid to the drain pan. All materials that come into contact with spilled materials should be disinfected and/or autoclaved.

A:TRUE

B:FALSE

Correct answer：

A

Question 15

Students should use surgical instruments in strict accordance with the requirements and specifications in the experiment. Pay attention to the safety of the surgical instruments. It is strictly forbidden to use surgical instruments for things unrelated to the experiment.

A:TRUE

B:FALSE

Correct answer：

A

Question 16

In animal experiments, students should pay attention to keeping the head faced away when grasping the sputum; do not squeeze the posterior glands of the ear in order to prevent the secretions of the glands behind the ear from entering the eyes of the experimenter. In case of being infected, the eyes should be rinsed immediately with saline.

A:TRUE

B:FALSE

Correct answer：

A

Question 17

All plastic utensils (eppendorf tubes, tips, etc.) used in genetic engineering operations should be sterilized in boxes and bottles before use, and should be placed in a box or bottling process with tweezers or disposable gloves. Operation, can not be directly taken by hand, to prevent contamination of the enzymes in the hands.

A:TRUE

B:FALSE

Correct answer：

A

Question 18

In the microbial experiment, there must be a "bacterial concept" and "aseptic operation awareness". The operation must be strictly aseptically operated according to the correct procedure, on the one hand to avoid infection, on the other hand to strengthen self-protection.

A:TRUE

B:FALSE

Correct answer：

A

Question 19

If there is an accident in the microbiological experiment (such as the broken seed tube), the disinfectant (84 disinfectant) should be used immediately to clean the table, wash hands, etc., in time to kill bacteria and viruses, to avoid the expansion of the contaminated surface.

A:TRUE

B:FALSE

Correct answer：

A

Question 20

After each microbial experiment, the desktop of the disinfection experiment must be cleaned and thoroughly washed.

A:TRUE

B:FALSE

Correct answer：

A

Question 21

In biochemical experiments where rubber or plastic gloves are required, it is not necessary to check the gloves frequently for damage.

A:TRUE

B:FALSE

Correct answer：

B

Question 22

In microbial experiments, some containers that are contaminated or contain harmful bacteria and viruses must be disinfected and autoclaved before reuse.

A:TRUE

B:FALSE

Correct answer：

A

Question 23

When the centrifuge is used up, the centrifuge lid can be opened without waiting for the speed display on the operator panel to be zero.

A:TRUE

B:FALSE

Correct answer：

B

Question 24

When the refrigerated centrifuge is used up, do not close the lid of the centrifuge immediately. It should be open for a period of time. After the frost caused by freezing is naturally dried, close the lid of the centrifuge.

A:TRUE

B:FALSE

Correct answer：

A

Question 25

During the fermentation experiment, it must be carried out in strict accordance with the operating procedures. Pay attention to the safety of water, electricity and steam (gas), strictly save, and strictly prohibit leakage. Be careful not to drop liquid such as acid and alkali onto the surface of the instrument to avoid contamination or corrosion of the surface of the instrument.

A:TRUE

B:FALSE

Correct answer：

A

Question 26

During the fermentation experiment, it is necessary to keep the indoor floor, table top, operation table and various items and utensils clean and hygienic, and also to keep the outside of the fermenter and its pipelines clean and free from dust and dirt.

A:TRUE

B:FALSE

Correct answer：

A

Question 27

In the "Animal Physiology Experiment", students should pay attention to keeping the head facing away when grasping the sputum, which can squeeze the glands behind the ear. If the glandular secretions of the ear are injected into the eyes of the experimenter, rinse the eyes with tap water.

A:TRUE

B:FALSE

Correct answer：

B

Question 28

After the end of the animal experiment, the experimental animals can be processed by themselves, and the undead animals can be discarded by hand.

A:TRUE

B:FALSE

Correct answer：

B

Question 29

When using a centrifuge, the balance of the centrifuged sample is not important.

A:TRUE

B:FALSE

Correct answer：

B

Question 30

All plastic utensils (eppendorf tubes, tips, etc.) used in the operation of Genetic Engineering Experiments should be sterilized in boxes and bottles before use, and can be directly packed or bottled by hand.

A:TRUE

B:FALSE

Correct answer：

B

Question 31

For biological experiments, disposable gloves are used. After the gloves are used up, they are bundled and discarded in a common garbage bin.

A:TRUE

B:FALSE

Correct answer：

B

Question 32

The eppendorf tube and tip used in the Molecular Biology Experiment are disposable articles, which can be discarded in ordinary garbage cans after use.

A:TRUE

B:FALSE

Correct answer：

B

Question 33

After the end of the fermentation experiment, the live cells can be discharged without heating and killing.

A:TRUE

B:FALSE

Correct answer：

B

Question 34

After each microbial experiment, the experimental tabletop must be cleaned with ordinary tap water and thoroughly washed.

A:TRUE

B:FALSE

Correct answer：

B

Question 35

In biochemical protein electrophoresis experiments, both monomeric acrylamide and methylidene diacrylamide are neurotoxic agents that are not absorbed by contact with the skin.

A:TRUE

B:FALSE

Correct answer：

B

Question 36

The carcinogens such as ethidium bromide (EB) and colchicine used in the experiment can be directly dumped into the water pipe or the garbage bin after the experiment.

A:TRUE

B:FALSE

Correct answer：

B

Question 37

In microbial experiments, some unwanted strains, etc., can be discarded directly without disinfection and autoclaving.

A:TRUE

B:FALSE

Correct answer：

B

Question 38

In microbiology experiments, some contaminated bacteria, pathogens, and discarded strains must be discarded after disinfection and autoclaving, and the sterilized vessels can be reused.

A:TRUE

B:FALSE

Correct answer：

A

Question 39

The laboratory should monitor the biosafety cabinet from time to time to ensure that its performance meets relevant requirements. The inspection record and any functional test results should be saved and the inspection certificate marked on the safety cabinet.

A:TRUE

B:FALSE

Correct answer：

A

Question 40

Sodium hypochlorite is generally not used as a bactericide, and is commonly used for general purpose disinfectants as well as for soaking contaminated non-metallic materials.

A:TRUE

B:FALSE

Correct answer：

A

Question 41

Formaldehyde can kill microorganisms and their spores and prions at temperatures above 20 °C.

A:TRUE

B:FALSE

Correct answer：

B

Question 42

A 75% aqueous solution of ethanol can be used to disinfect skin, laboratory bench surfaces, and small instruments. Disinfect the skin for at least 10 seconds and disinfect the surface of other objects for less than 3 minutes.

A:TRUE

B:FALSE

Correct answer：

A

Question 43

Before using the sterile room, all the items to be inoculated should be moved into the buffer room of the sterile room, and wiped clean with a 75% alcohol cotton ball. Finally, the items are sent to the workbench, and the UV lamp is turned on to disinfect the surface of the object for about 30 minutes.

A:TRUE

B:FALSE

Correct answer：

A

Question 44

After the use of the sterile room, clean up the items and turn on the fluorescent lamp for about 30 minutes.

A:TRUE

B:FALSE

Correct answer：

B

Question 45

Hydrogen peroxide and peracetic acid can be used to disinfect laboratory surfaces and metal instruments.

A:TRUE

B:FALSE

Correct answer：

B

Question 46

Gas fumigation is commonly used in the disinfection of biological laboratories, especially for the disinfection of rooms and equipment.

A:TRUE

B:FALSE

Correct answer：

A

Question 47

The gas fumigation method can be used for disinfecting the air in the biosafety cabinet, but it cannot be used for disinfecting the contents of the biosafety cabinet.

A:TRUE

B:FALSE

Correct answer：

B

Question 48

When filtering infectious substances with a glass filter, it should be immersed in 2% carbolic acid solution for at least 2 hours, then washed and autoclaved.

A:TRUE

B:FALSE

Correct answer：

A

Question 49

Before or after use of the biosafety cabinet, the biosafety cabinet should be operated for at least 5 minutes to complete the “purification” process, that is, the time for discharging the polluted air out of the biosafety cabinet should be set aside.

A:TRUE

B:FALSE

Correct answer：

A

Question 50

The operator should move slowly when entering and exiting the biosafety cabinet with both arms to maintain the integrity of the airflow at the opening of the operating surface.

A:TRUE

B:FALSE

Correct answer：

A

Question 51

After the experiment, the instruments, equipment and other items used in the biosafety cabinet may be contaminated, so they should be removed from the cabinet after disinfection.

A:TRUE

B:FALSE

Correct answer：

A

Question 52

During the use of the biosafety cabinet, for the convenience of experimentation, the equipment and articles in the cabinet should be replaced as often as possible.

A:TRUE

B:FALSE

Correct answer：

B

Question 53

During the use of the biosafety cabinet, for the convenience of operation, it should be operated at the front of the cabinet.

A:TRUE

B:FALSE

Correct answer：

B

Question 54

The test procedures for harmful microorganisms and genetically modified organisms are carried out in a biosafety cabinet.

A:TRUE

B:FALSE

Correct answer：

A

Question 55

When carrying out pathogenic microbiology experiments, the biosafety cabinet can be replaced by a super clean bench.

A:TRUE

B:FALSE

Correct answer：

B

Question 56

In order to prevent contamination, some experiments require wearing disposable gloves. So wear gloves when you enter the lab and always wear everything.

A:TRUE

B:FALSE

Correct answer：

B

Question 57

The drying oven (oven) can be used for drying, dry heat sterilization of the articles, and the incubator is used for microbial culture and biological material culture.

A:TRUE

B:FALSE

Correct answer：

A

Question 58

The environmental conditions of the general laboratory cannot meet the national standards of animal laboratories and cannot be used as a venue for animal experiments.

A:TRUE

B:FALSE

Correct answer：

A

Question 59

Laboratory animal practitioners and animal experimenters infected with chronic infectious diseases such as hepatitis B, should not be exposed to experimental animals and engage in related work.

A:TRUE

B:FALSE

Correct answer：

A

Question 60

Different grades of experimental animals must be kept in the appropriate level of facilities.

A:TRUE

B:FALSE

Correct answer：

A

Question 61

If the animal laboratory is equipped with a variety of excellent facilities and equipment, it can be relaxed in terms of implementation of regulations and operating procedures.

A:TRUE

B:FALSE

Correct answer：

B

Question 62

Normal animal laboratories emphasize that items entering the room must be rigorously sterilized, while infected animal laboratories emphasize that items that enter and exit must undergo strict high temperature sterilization.

A:TRUE

B:FALSE

Correct answer：

A

Question 63

The Regulations on Biosafety Management of Pathogenic Microorganisms, promulgated by the State Council on November 12, 2004, are the main regulations on which the laboratory biosafety management system is established.

A:TRUE

B:FALSE

Correct answer：

A

Question 64

Animal experiments can be carried out, and experimental animals such as rabbits can be purchased at the farmer market.

A:TRUE

B:FALSE

Correct answer：

B

Question 65

After the animal experiment, the dead animal carcasses and organs can be reused as feed ingredients.

A:TRUE

B:FALSE

Correct answer：

B

Question 66

Isotopic laboratory managers have the right to refuse entry to the isotope room without training, except for teachers.

A:TRUE

B:FALSE

Correct answer：

B

Question 67

To purchase a radionuclide, you must apply to the person in charge of the isotope laboratory for registration. When purchasing, receiving, using, and returning radioisotopes, they should be properly registered and carefully checked to ensure that the accounts are consistent.

A:TRUE

B:FALSE

Correct answer：

A

Question 68

Experimental procedures for radionuclides must be performed at designated isotope laboratories.

A:TRUE

B:FALSE

Correct answer：

A

Question 69

All wastes and animal carcasses generated by the radioisotope laboratory must be effectively decayed and monitored by isotope radiation before they can be disposed of in accordance with general waste and animal carcasses.

A:TRUE

B:FALSE

Correct answer：

A

Question 70

The liquid waste generated by the radioisotope laboratory can be sealed and stored in a temporary transfer station for chemical waste.

A:TRUE

B:FALSE

Correct answer：

B

Question 71

Acid tanks for soaking glassware must have a protective cover and must not be left open.

A:TRUE

B:FALSE

Correct answer：

A

Question 72

Small rodents used in experimental studies are generally free of pathogenic infections and you do not have to look at their biosafety issues during the course of the experiment.

A:TRUE

B:FALSE

Correct answer：

B

Question 73

Experimental animal biosafety events are mainly manifested in two aspects. First, normal animals in the process of infection, infection with human and animal diseases; second, infected animals due to escape or improper disposal of waste, etc., resulting in the spread of infectious diseases.

A:TRUE

B:FALSE

Correct answer：

A

Question 74

Contaminated (infected) sharp objects, such as injection needles, anatomical blades and broken glass; should be placed in a covered container that is not easily punctured and treated as an infectious substance.

A:TRUE

B:FALSE

Correct answer：

A

Question 75

Experimentally discarded bioactive experimental materials, especially cells and microorganisms (bacteria, fungi, viruses, etc.) must be inactivated and disinfected in a timely manner. Solid media and the like should be autoclaved, and solid waste that has not been effectively treated cannot be disposed of as daily garbage.

A:TRUE

B:FALSE

Correct answer：

A

Question 76

The culture to be disposed of and the contaminated material can be disposed together with the domestic waste.

A:TRUE

B:FALSE

Correct answer：

B

Question 77

Bio-contaminated liquids must be cleaned up before being discharged into domestic sewage pipes.

A:TRUE

B:FALSE

Correct answer：

A

Question 78

The biosafety level laboratory must be constructed in accordance with national regulations, and the biosafety level (BSL-1) and second level (BSL-2) laboratories should be filed with the provincial competent authority, Biosafety Level 3 (BSL-3), Level 4 (BSL-4) laboratories must obtain nationally recognized qualifications.

A:TRUE

B:FALSE

Correct answer：

A

Question 79

Research on the development of highly pathogenic microorganisms must be carried out in qualified biosafety Level 3 and Level 4 laboratories, and the projects carried out must be submitted to the provincial health and agricultural authorities for review and approval. Other research work on pathogenic microorganisms must be carried out in primary and secondary biosafety laboratories.

A:TRUE

B:FALSE

Correct answer：

A

Question 80

Biosafety Level 3 and Level 4 laboratories are not allowed to engage in high pathogenic microorganisms.

A:TRUE

B:FALSE

Correct answer：

B

Question 81

The anesthetic used by the students in the experiment is uniformly prepared by the teacher, and the unused anesthetic is uniformly handled by the teacher, and the students are not allowed to discard them at will.

A:TRUE

B:FALSE

Correct answer：

A

Question 82

It is necessary to standardize the procurement procedures, experimental operations, waste disposal and other work procedures for biochemical reagents and supplies, and strengthen the safety management of biological laboratories.

A:TRUE

B:FALSE

Correct answer：

A

Question 83

Biological laboratory waste (including animal residues, etc.) is collected in a special container and subjected to high temperature and high pressure sterilization. Disposable gloves and items contaminated with EB carcinogens in biological experiments should be collected and disposed of in a unified manner and should not be disposed of in ordinary bins.

A:TRUE

B:FALSE

Correct answer：

A

Question 84

In microbial experiments, some unwanted strains must be discarded after disinfection and autoclaving.

A:TRUE

B:FALSE

Correct answer：

A

Question 85

The biological laboratory can cook food, gather meals, and students can stay in the lab.

A:TRUE

B:FALSE

Correct answer：

B

Question 86

Students entering the biochemical and pharmaceutical laboratory may not wear a uniform laboratory suit (Bai Dawei).

A:TRUE

B:FALSE

Correct answer：

B

Question 87

Workers should wear back-open work clothes or other protective clothing in a dedicated locker room (or buffer room) before entering the BSL-2 or higher laboratory work area. Work clothes must be taken off, and work clothes must not be taken out of the laboratory. Work clothes that can be reused must be sterilized and cleaned.

A:TRUE

B:FALSE

Correct answer：

A

Question 88

When laboratory activities involve infectious or potentially infectious biological factors, no assessment of the extent of the hazard is required.

A:TRUE

B:FALSE

Correct answer：

B

Question 89

Operators entering the laboratory for the first time should be aware of the specific hazards of the laboratory and read and understand the safety manual and operating manual carefully.

A:TRUE

B:FALSE

Correct answer：

A

Question 90

Any dead animal carcass or tissue fragments in the biomedical laboratory must be autoclaved and sealed in a plastic bag for special treatment.

A:TRUE

B:FALSE

Correct answer：

A

Question 91

According to the degree of hazard of the biological factors being operated and the protective measures to be taken, the level of biosafety protection is divided into four levels; the level 1 protection level is the lowest, and the level 4 protection level is the highest. The corresponding biosafety protection levels of the laboratory are indicated by BSL-1, BSL-2, BSL-3, and BSL-4.

A:TRUE

B:FALSE

Correct answer：

A

Question 92

Operators must wash their hands and not disinfect after contact with infectious materials and animals.

A:TRUE

B:FALSE

Correct answer：

B

Question 93

Experiments were carried out in the BSL-1, BSL-2, BSL-3, and BSL-4 laboratories according to the degree of pathogenic microorganism damage involved in the experiment, the experimental project, whether the material was inactivated, and whether the specimen was cultured.

A:TRUE

B:FALSE

Correct answer：

A

Question 94

In the biological laboratory, you can open the door, cabinet door and refrigerator door with gloves.

A:TRUE

B:FALSE

Correct answer：

B

Question 95

Formaldehyde vapor has a strong stimulating effect on humans. After the fumigation is completed, immediately open the doors and windows, and put the same amount of ammonia into the room to reduce the irritating effect on people.

A:TRUE

B:FALSE

Correct answer：

A

Question 96

Ethidium bromide (EB), which is exposed to experiments in molecular biology, genetics, genetic engineering, etc., is a carcinogen and you must not allow the drug to come into contact with the skin during the experiment.

A:TRUE

B:FALSE

Correct answer：

A

Question 97

The colchicine that is exposed to experiments in genetics is a carcinogen, and a small amount of the drug can be exposed to the skin during the experiment.

A:TRUE

B:FALSE

Correct answer：

B

Question 98

In biochemical protein electrophoresis experiments, both monomeric acrylamide and methylidene diacrylamide are neurotoxic agents that are absorbed in contact with the skin. Care should be taken when performing experiments. In case of contact, rinse immediately with running water for 5 minutes.

A:TRUE

B:FALSE

Correct answer：

A

Question 99

Effective disinfectants, eye cleansers or saline must be available in biological laboratories and are readily available. Can be equipped with emergency medicine.

A:TRUE

B:FALSE

Correct answer：

A

Question 100

H2S is colorless, smells bad, is hard to dissolve in water, inhibits the activity of enzymes, and suffocates tissue.

A:TRUE

B:FALSE

Correct answer：

B

Question 101

The BSL-2 laboratory refers to a clean, sterile room.

A:TRUE

B:FALSE

Correct answer：

B

Question 102

In the "Genetics Experiment" fruit fly experiment, diethyl ether was repeatedly used, and the ether was not flammable and explosive.

A:TRUE

B:FALSE

Correct answer：

B

Question 103

The assessment of the degree of hazard mainly includes the type, source, infectivity, pathogenicity, route of transmission, stability in the environment, dose of infection, concentration, animal experimental data, prevention and treatment of biological factors.

A:TRUE

B:FALSE

Correct answer：

A

Question 104

Biological factors are all microorganisms and biologically active substances.

A:TRUE

B:FALSE

Correct answer：

A

Question 105

Chemical bactericides are chemicals or mixtures of chemicals used to kill microorganisms, including their spores.

A:TRUE

B:FALSE

Correct answer：

A

Question 106

Disinfectants are chemicals or a mixture of several chemicals that can kill microorganisms, including their spores.

A:TRUE

B:FALSE

Correct answer：

B

Question 107

Sterilization refers to the physical or chemical killing of all microorganisms and their spores on an object.

A:TRUE

B:FALSE

Correct answer：

A

Question 108

Wet heat sterilization is performed by the action of heat, usually in a high pressure steam sterilizer.

A:TRUE

B:FALSE

Correct answer：

A

Question 109

The maximum temperature for moist heat sterilization is usually 121 ° C for 15 min.

A:TRUE

B:FALSE

Correct answer：

B

Question 110

Dry heat sterilization is performed by the action of heat, usually in a dry heat sterilizer; the use temperature is usually 160-400 ° C, and the time is 1-5 h.

A:TRUE

B:FALSE

Correct answer：

A

Question 111

Compared with dry heat sterilization, dry (same?) heat sterilization has strong penetrating power and fast conduction.

A:TRUE

B:FALSE

Correct answer：

B

Question 112

Both moist heat sterilization and dry heat sterilization have their own characteristics, but in general, dry heat sterilization is better than moist heat sterilization.

A:TRUE

B:FALSE

Correct answer：

B

Question 113

The higher the concentration of the aqueous ethanol solution, the stronger the bactericidal activity.

A:TRUE

B:FALSE

Correct answer：

B

Question 114

Biosafety mainly refers to the adoption of appropriate protective facilities, equipment, protective principles and technical operations in the course of biological research and teaching to reduce or eliminate the exposure and accidental release of potentially dangerous factors, thereby ensuring human and environmental safety; It also includes preventing pathogens or toxins and related information from being stolen and abused by criminals such as terrorists.

A:TRUE

B:FALSE

Correct answer：

A

Question 115

Regarding ultraviolet disinfection, since the visible light can revitalize the photoreactivation enzyme in the living body, the formed dimer is taken apart and restored, so that the fluorescent lamp and the ultraviolet lamp cannot be turned on at the same time.

A:TRUE

B:FALSE

Correct answer：

A

Question 116

Disposable items, including syringes and needles, can be reused after disinfection.

A:TRUE

B:FALSE

Correct answer：

B

Question 117

The gas, alcohol, gasoline, etc. in the biological laboratory are inflammable and explosive fuels, which can cause burning and explosion under certain conditions, and must be properly placed and used correctly.

A:TRUE

B:FALSE

Correct answer：

A

Question 118

Ether, xylene, acetone, trinitrobenzenesulfonic acid, turpentine, picric acid, etc. in the biological laboratory are inflammable and explosive liquids. Under certain conditions, they can cause burning and explosion. They must be properly placed and used correctly. .

A:TRUE

B:FALSE

Correct answer：

A

Question 119

Rosin, sulfur, inorganic phosphorus, etc. in the biological laboratory are flammable and explosive solids, which can cause burning and explosion under certain conditions, and must be properly placed and used correctly.

A:TRUE

B:FALSE

Correct answer：

A

Question 120

Students can eat chewing gum when they are in a biological laboratory.

A:TRUE

B:FALSE

Correct answer：

B

Question 121

Ethanol acts on active bacteria, fungi, and lipid viruses, but has no effect on spores.

A:TRUE

B:FALSE

Correct answer：

A

Question 122

The isotope P-32 and S-35 can be placed together, but H-3, I-125, and C-14 must be placed separately.

A:TRUE

B:FALSE

Correct answer：

A

Question 123

The water vapor has latent heat. When the water vapor comes into contact with the sterilized article, it can be condensed into water to release latent heat, so that the temperature is rapidly increased and the sterilization effect is enhanced.

A:TRUE

B:FALSE

Correct answer：

A

Question 124

Visible light can revitalize the photoreactivation enzyme in the organism and disassemble the formed dimer. Therefore, when using ultraviolet light sterilization, the fluorescent lamp and the ultraviolet lamp cannot be turned on at the same time.

A:TRUE

B:FALSE

Correct answer：

A

Question 125

UV disinfection is convenient and practical, but it cannot be relied upon for complete sterilization, especially for bacterial spore killing.

A:TRUE

B:FALSE

Correct answer：

A

Question 126

Due to the poor ability of UV to penetrate substances, it is not suitable for indoor air or surface disinfection.

A:TRUE

B:FALSE

Correct answer：

B