Fluoca-Olympus TIRF microscope
Fluoca-Olympus TIRF microscope
Instrument category： Image Core
Instrument mode：
Unit： 浙江大学 > ZJU-UoE Institute
Instrument manufacturer： Fluoca
Purchase date： 2019-09-28
Usage patterns： Sample presentation reservation,Machine-time reservation
Specification： Olympus fcTIRF
Room number：A121
Collection
Instrument manufacturer： Fluoca
Asset number： 21008870
Asset manager： 贾玲燕
Purchase date： 2019-09-28
Instrument price： 98.00 Ten thousand yuan
Origin of instruments： Japan
Instrument supplier： Olympus
Purchase manager： Lin Jiangtao
Main accessories： Laser light source: 488nm, 561nm Objective lens: 10X, 20X, 40X, 60X Camera: Andor iXon DU897 EMCCD camera
Main parameter： ----
Introduction of instruments：
Total internal reflection fluorescence (TIRF) microscopy, also known as evanescent wave/field microscopy, is a technique that uses a special mode of sample illumination to exclusively excite fluorophores that are within approximately 100 nm of the cover glass/sample interface. This mode of illumination is based on an evanescent field which is produced when light rays are totally internally reflected at the cover glass/substrate interface. The evanescent field does not propagate deeper into the sample; therefore, images are not contaminated with fluorescence from out-of-focus planes. As a result, TIRF microscopy provides a means of imaging fluorophores near the cover glass/substrate interface with a high signal-to-noise ratio, thus making it the current gold standard technique for studying events that occur at the adherent plasma membrane of cells.
| Working hours： | 09:00-12:00;14:00-18:00 |
|---|---|
| Minimum reservation time interval： | 0.5 Hours |
| Maximum reservation time interval： | 168 Hours |
| Minimum unit time of calendar： | 0.5 Hours |
| Latest reservation time： | Unauthorized user: 0Hours； General qualified user: 0Hours； Senior qualified user: 0Hours |
| Earlist reservation time： | Unauthorized: 8 Week 0 o'clock； Ordinary: 8 Week 0 o'clock； Senior: 8 Week 0 o'clock |
| Reservation protection time： | 15 minutes |
| No-show protection time： | 15 minutes |
| Outage delay time： | 30 Seconds |
| Minimum power-on time： | 5 Seconds |
| Timeout reminder： | 1200 Seconds |
| Maximum effective number of reservations： | 5 次/ Day |
| Costless revocation of reservation time： | 120 minutes |
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Main instrument manager： 刘娣(邮箱:diliu@intl.zju.edu.cn) Instrument manager： 沈杭晨(邮箱:shc14124818@163.com) Instrument manager： 贾玲燕(邮箱:lingyanjia@intl.zju.edu.cn) Instrument manager： 王依依(邮箱:21716013@zju.edu.cn) Instrument manager： 金梦迪(邮箱:mengdijin@intl.zju.edu.cn) Instrument manager： 朋德测试(邮箱:11111@qq.com) Instrument manager： 蔡政(邮箱:675097016@qq.com) Instrument manager： 李媛(邮箱:yuanli@intl.zju.edu.cn) Instrument manager： 朱乐(邮箱:11616028@zju.edu.cn) |
| User qualification | Working hours | Non working time |
|---|---|---|
| Unauthorized qualification | Need to review | Need to review |
| General qualification | Avoid audit | Avoid audit |
| Senior qualification | Avoid audit | Avoid audit |
| No. | Title | Creation time | Operation |
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| No. | Title | File number | Creation time | Operation |
|---|---|---|---|---|
| 1 | user guide | 3 | 2021-01-11 21:39:42 | Download files |
| Title | Training Video | Creation time | Operation |
|---|