Instrument category： Image Core
Instrument mode：
（肖曼瑜）Unit： 浙江大学 > ZJU-UoE Institute
Instrument manufacturer： Zeiss
Purchase date： 2019-12-14
Usage patterns： Machine-time reservation
Specification： LSM 880 (Zeiss)
Room number：A119-2
Zeiss LSM 880 Confocal Microscope
Zeiss LSM 880 Confocal Microscope
Instrument category： Image Core
Instrument mode：
（肖曼瑜）Unit： 浙江大学 > ZJU-UoE Institute
Instrument manufacturer： Zeiss
Purchase date： 2019-12-14
Usage patterns： Machine-time reservation
Specification： LSM 880 (Zeiss)
Room number：A119-2
Collection
Instrument manufacturer： Zeiss
Asset number： 20002656
Asset manager： ----
Purchase date： 2019-12-14
Instrument price： 390.00 Ten thousand yuan
Origin of instruments： Germany
Instrument supplier： ----
Purchase manager： ----
Main accessories： Airyscan, Living cell workstation, Compensation module Definite Focus, Scanning Stage 130x100 STEP Set LSM, User-PC advanced Z55A Highend
Main parameter： 1. Light sources: Diode laser – 405 nm； Argon laser – 458 nm, 488 nm, 514 nm； DPSS – 561 nm； HeNe – 633 nm； 2. Objiectives: 10X, NA 0.45; 20X, NA 0.80; 40X, NA 1.40; 63X, NA 1.40; 100X NA 1.40. 3. Detection source: 2 x Big (GaAsP) NDD detectors； 2 x NDD detectors； 1 x 32 channel GaAsP array； 1 x Airyscan Detector.
Introduction of instruments：
LSM 880 with Airyscan
LSM 880 lets the user take full advantage of large fields of view and the highest speed of any linear scanning confocal microscope at an unsurpassed image quality. Designed for optimized productivity, LSM 880 identifies and localizes biological molecules of interest even in the most challenging specimens. To investigate subcellular structures at a new level of detail, the system provides the speed to monitor these molecules and their interactions in space and time while minimizing the input of light.
1.Available modalities
Z-stack
Time-series
Tile scan
Multi positions
FRAP
2.Additional hardware
Airyscan detection
Fast Airyscan module
| Working hours： | 08:30-11:30;13:00-17:00 |
|---|---|
| Minimum reservation time interval： | 0.5 Hours |
| Maximum reservation time interval： | 168 Hours |
| Minimum unit time of calendar： | 0.5 Hours |
| Latest reservation time： | Unauthorized user: 0Hours； General qualified user: 0Hours； Senior qualified user: 0Hours |
| Earlist reservation time： | Unauthorized: 8 Week 0 o'clock； Ordinary: 8 Week 0 o'clock； Senior: 8 Week 0 o'clock |
| Reservation protection time： | 15 minutes |
| No-show protection time： | 15 minutes |
| Outage delay time： | 30 Seconds |
| Minimum power-on time： | 5 Seconds |
| Timeout reminder： | 120 Seconds |
| Maximum effective number of reservations： | 10 次/ Day |
| Costless revocation of reservation time： | 120 minutes |
平台预约
|
Main instrument manager： 刘娣(邮箱:diliu@intl.zju.edu.cn) Instrument manager： 沈杭晨(邮箱:shc14124818@163.com) Instrument manager： 贾玲燕(邮箱:lingyanjia@intl.zju.edu.cn) Instrument manager： 王依依(邮箱:21716013@zju.edu.cn) Instrument manager： 金梦迪(邮箱:mengdijin@intl.zju.edu.cn) Instrument manager： 朋德测试(邮箱:11111@qq.com) Instrument manager： 蔡政(邮箱:675097016@qq.com) Instrument manager： 李媛(邮箱:yuanli@intl.zju.edu.cn) Instrument manager： 朱乐(邮箱:11616028@zju.edu.cn) |
| User qualification | Working hours | Non working time |
|---|---|---|
| Unauthorized qualification | Need to review | No reservation |
| General qualification | Avoid audit | No reservation |
| Senior qualification | Avoid audit | Avoid audit |
| No. | Title | Creation time | Operation |
|---|---|---|---|
| 1 | Operation assessment reference of LSM880 confocal microscope | 2024-02-26 10:13:52 | Detail |
| No. | Title | File number | Creation time | Operation |
|---|---|---|---|---|
| 1 | Zeiss 880 microscope application and principle introduction | 1 | 2022-05-23 10:12:20 | Download files |
| 2 | Zeiss 880 microscope Basic operation | 1 | 2022-05-23 09:53:39 | Download files |
| Title | Training Video | Creation time | Operation |
|---|---|---|---|
| FRET analysis | 1702456106iznqi.mp4 | 2023-12-13 16:28:51 | Detail |
| FRET sensitized emission | 1702456051mqmuq.mp4 | 2023-12-13 16:27:51 | Detail |
| FRET acceptorphoto bleach | 1702456008krgqr.mp4 | 2023-12-13 16:27:01 | Detail |
| FRAP analysis | 1702455956oimkd.mp4 | 2023-12-13 16:26:00 | Detail |
| FRAP imaging | 1702455897ytbaq.mp4 | 2023-12-13 16:25:15 | Detail |
| Colocalization | 1702455776zycvr.mp4 | 2023-12-13 16:24:20 | Detail |
| Time series | 1702454410dshhk.mp4 | 2023-12-13 16:00:22 | Detail |
| Tile scan | 1702454368bsvix.mp4 | 2023-12-13 15:59:48 | Detail |
| [3] Image save and export | 1702454316ivuws.mp4 | 2023-12-13 15:58:54 | Detail |
| [4] Airyscan imaging | 1702454281ypbrs.mp4 | 2023-12-13 15:58:13 | Detail |
| [4] Airyscan fast imaging | 1702454228llekd.mp4 | 2023-12-13 15:57:29 | Detail |
| [2] 880 Z-stack imaging | 1702452692cdkyc.mp4 | 2023-12-13 15:33:49 | Detail |
| [1] 880简单操作步骤 | 1702432349gajdx.mp4 | 2023-12-13 09:52:32 | Detail |